ISHS Acta Horticulturae 795: V International Cherry Symposium
IN VITRO PROPAGATION OF THE P-HL DWARFING SWEET CHERRY ROOTSTOCKS |
| Authors: | J. Sedlak, F. Paprstein, M. Erbenova |
| Keywords: | Prunus avium, micropropagation, culture initiation, multiplication, 6-benzylaminopurine, cefotaxime |
| Abstract:
We have developed a rapid shoot multiplication procedure for in vitro propagation of dwarfing P-HL rootstocks (‘P-HL A’, ‘P-HL B’, ‘P-HL C’) for sweet cherries (Prunus avium L.). Shoot tips were successfully established in vitro and micropropagated on Murashige and Skoog (MS) based media containing different concentrations of 6-benzylaminopurine (BAP). Multiplication rates varied depending on the rootstock and concentration of BAP. The highest multiplication rate was obtained for ‘P-HL A’, yielding 10.9±0.5 shoots (>10 mm) on MS medium containing 1.5 mg L-1 BAP. The lowest multiplication rate was obtained for ‘P-HL B’, which yielded only 2.0±0.3 shoots on MS medium containing 0.2 mg L-1 BAP. The highest concentration of BAP (2 mg L-1) was deleterious to shoot growth and multiplication rate. The antibiotic cefotaxime (200 mg L-1) eliminated bacterial contamination during in vitro multiplication. Addition of indole-3-butyric acid (IBA) improved the in vitro rooting on modified MS medium. | |
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