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| Authors: | A. Fidancı, M. Baş, M. Burak |
| Keywords: | Prunus avium, sterilization, breeding, seedling survival |
Abstract:
To obtain self-fertile and high quality sweet cherry cultivars, a breeding program was initiated at Yalova-Atatürk Central Horticultural Research Institute.
Although the hybrid seeds were stratified conventionally, none germinated.
To solve this problem, different stratification methods were investigated.
Embryo rescue was selected for obtaining new plants from the hybridizations; however, while germination percentage was high, the survival rate of the seedlings was very low.
Another new method was applied as follows: hybrid seeds were taken from the orchard after fruit maturation and seeds were disinfected.
After surface sterilization, seeds were put in large vessels, double distilled water was added, and the water was changed every day.
Ten days later, the seed coats were removed and the seeds were placed in vessels containing wet perlite under sterile conditions.
The vessels were kept in a refrigerator at 4°C for two months.
Germinated seeds were sown in small plastic pots containing peatmoss under greenhouse conditions.
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