ISHS Acta Horticulturae 793: XI International Workshop on Fire Blight
POTENTIAL OF SURFACE PLASMON RESONANCE (SPR) FOR THE RAPID IDENTIFICATION OF ERWINIA AMYLOVORA |
| Authors: | J.L. Vanneste, J. Yuan, J. Yu, Y. Wu |
| Keywords: | BIAcore, fire blight |
| Abstract:
Surface Plasmon Resonance (SPR) allows real-time detection of biomolecular binding events. It has been used for the specific detection of several food-borne bacterial pathogens based on the particular interactions between antigens and monoclonal antibodies. Only very few studies took advantage of DNA specificity for the detection of a pathogen. In this study, presence of Erwinia amylovora, the fire blight pathogen, was detected by injecting a solution containing a 25 mer DNA fragment specific of this bacterium into a BIAcore biosensor machine loaded with a 25 mer complementary DNA fragment. If the polymerase chain reaction (PCR) step used to generate the target DNA could be omitted, then detection of E. amylovora in plant tissues could be carried out in a continuous fully automated operation. | |
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