ISHS Acta Horticulturae 791: V International Symposium on Olive Growing
IDENTIFICATION OF IRANIAN OLIVE CULTIVARS BY USING RAPD AND MICROSATELLITE MARKERS |
| Authors: | M. Shahriari, A. Omrani, M. Falahati-Anbaran, B. Ghareyazei, A. Nankali |
| Keywords: | olive (Olea europaea L.), microsatellite markers, identification, inter-intra cultivar genetic diversity, analysis of molecular variance |
| Abstract:
Sixteen microsatellite loci were used to identify and investigate the inter and intra cultivar genetic variation and relationships of Iranian and some imported olive (Olea europaea L.) cultivars. All microsatellite loci were polymorphic, except GAPU14 and GAPU113, which were monomorphic. Totally 233 different genotypes obtained by 120 polymorphic alleles observed at 14 polymorphic loci in 55 olive accessions. The average number of alleles per locus was 8.57, ranging from 3 to 14. 23.1% of all possible genotypes were homozygous revealing 32.5% total of accessions. RAPD analysis primary performed on Roudbar and Zandjan accessions using 13 arbitrarily primers. 176 out of 195 amplified fragments produced by RAPD were polymorphic. Due to low reliability of RAPD results, we didn’t continue. The power of discrimination (PD) was 0.82. The genetic similarity based on Jaccard coefficient ranged from 0.14 to 1. The genetic relationships among accessions were investigated using cluster analysis and principal component analysis (PCA). All of accessions belonging to same cultivar were different from each other, except Shenge (D) and Shenge (A) which were similar together. A close relationship was observed among accessions within same cultivar. Most of the Iranian olive accessions clustered to two main distinct groups. Syrian cultivars were also separated from two parts of Iranian accessions indicating that different region of origination of them. Two cultivars, Molasani (A) and Kalamata (R), with unique morphological characters, clustered far from the other accessions. To partitioning genetic diversity within and among cultivars (including 3 or more accessions), analysis of molecular variance (AMOVA) revealed higher genetic variation within cultivars (64.42%) than among (35.58%) them. The inter-intra cultivar variation was significant statistically. Clustering of accessions belonging to same cultivar suggested the existence of related genetic base with little morphological differences strictly. | |
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