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ISHS Acta Horticulturae 789: XV Meeting of the EUCARPIA Tomato Working Group

VARIATION IN TOMATO PLANTS REGENERATED FROM CUCUMBER MOSAIC VIRUS INFECTED TISSUE

Author:   E. Hanus-Fajerska
Keywords:   intermediate organogenesis, somaclonal variation, viral infection
Abstract:
Experiments were carried out to check the effect of a Lycopersicon esculentum Cucumber mosaic virus (CMV) model on traits connected with generative propagation of somaclones obtained from virus – infected tissue. Specific pathogen-free regenerants obtained through indirect organogenesis from CMV-infected tissue were compared to regenerants obtained from healthy control plants and to standard plants obtained from seed. Regenerated plants, after acclimatization to ex vitro conditions, were cultivated in greenhouse. Variability of somaclones obtained from healthy material in regard to all evaluated traits was lower than that observed in specific pathogen-free somaclones. Significant differences between the two tomato cultivars were proven for pollen viability and fruit set.

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