ISHS Acta Horticulturae 789: XV Meeting of the EUCARPIA Tomato Working Group
BREEDING FOR RESISTANCE TO TOMATO YELLOW LEAF CURL DISEASE (TYLCD) AT THE INSTITUTE FOR THE CONSERVATION AND IMPROVEMENT OF AGRODIVERSITY (COMAV) |
| Authors: | A. Pérez de Castro, M.J. Díez, F. Nuez |
| Keywords: | Lycopersicon spp., inoculation methods, molecular hybridization, transgenic plants |
| Abstract:
Tomato yellow leaf curl disease (TYLCD) is one of the main threats to tomato crops in southern Spain and in many temperate areas of the world. Several viral species have been described as causal agents of TYLCD. Genetic resistance seems to be the best long-term strategy to control the damage caused by this viral disease. Breeding for resistance to TYLCD is one of the areas of research at the Institute for the Conservation and Improvement of Agrodiversity (COMAV). The main objective is to integrate strategies aimed at controlling TYLCD. We use two different approaches: host-derived resistance and pathogen-derived resistance. Given that all tomato cultivars are susceptible to TYLCD, screening for resistance has focused on wild Lycopersicon species, such as L. pimpinellifolium (Jusl.) Mill., L. hirsutum Humb. & Bonpl., L. peruvianum (L.) Mill., L. chilense Dun., L. pennellii (Corr.) D'Arcy and L. esculentum var. cerasiforme Mill. (Dunal) Gray. Inoculation techniques and diagnostic methods have been previously established. Among the accessions tested, the highest levels of resistance have been found in L. chilense, and breeding programs for the introgression of the resistance found in this species have been initiated; however, resistance to this disease is always a partial resistance, so it would be useful to make use of the resistance found in different species, since the accumulation of genes from diverse sources could lead to a more durable resistance. High levels of resistance were also identified in some accessions of L. peruvianum and L. pimpinellifolium. The genetic control of the resistance found in these accessions is being studied. Regarding pathogen-derived resistance, the C1 gene in antisense and iRNA strategies are being used. Transgenic tomato plants have been obtained. The molecular characterization and evaluation of the resistance in these plants are under way. | |
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