ISHS Acta Horticulturae 786: International Workshop on Medicinal and Aromatic Plants
ANTIMICROBIAL EFFECTS OF SOME MEDICINAL PLANT EXTRACTS AGAINST BACTERIA ASSOCIATED WITH BLACK DISEASE |
| Authors: | N. Muangsan, V. Senamontee |
| Keywords: | fairy shrimps, inhibition zones, agar well diffusion method, minimal inhibitory concentration, ethanolic extract |
| Abstract:
The objective of this study is to determine the potential antimicrobial activity of water and ethanolic extracts from five medicinal plants against bacteria isolated from diseased fairy shrimps. The antimicrobial efficacy of Andrographis paniculata, Garcinia mangostana, Psidium guajava, Punica granatum and Terninalia catappa was evaluated against four gram negative bacteria including Aeromonas sobria, Klebsiella pneumoniae, Enterobacter sp. and Chryseobacterium sp. using agar well diffusion method at three concentration levels (100, 10, and 1 mg/ml). Among the tested plants, P. granatum extract showed the highest antibacterial activity against all four bacterial strains at 10 mg/ml. In addition, almost all species of plants were found to have activity on at least two microbial strains. However, the diameters of inhibition zone were different according to the kinds and concentrations of plant extracts and bacterial strains. The minimal inhibitory concentration (MIC) of both water and ethanolic extract of P. granatum and ethanolic extract of T. catappa were highly effective against all bacterial strains tested with the MICs ranging from 3.125 mg/ml to 25 mg/ml. The ethanolic extract of P. granatum was very active against A. sobria at a concentration of 3.125 mg/ml. | |
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