ISHS Acta Horticulturae 786: International Workshop on Medicinal and Aromatic Plants
INTRASPECIFIC VARIABILITY ASSESSMENT OF ANDROGRAPHIS PANICULATA COLLECTIONS USING MOLECULAR MARKERS |
| Authors: | S. Sakuanrungsirikul, A. Jetana, P. Buddanoi, J. Dithachaiyawong |
| Keywords: | andrographolide, antipyretic, primers, monomorphic, polymorphic, ISSR, RAPD |
| Abstract:
Andrographis paniculata is a traditional medicinal herb with antipyretic and other curative properties. The main active ingredients are in the lactone group including, andrographolide, deoxy-andrographolide and neoandro-grapholine. Forty four accessions of A. paniculata were collected from nine distribution areas throughout Thailand to seek clones with high active ingredients beneficial for production of this natural drug. Genetic differentiation was done in S2 generation using two different marker approaches; 1) ISSR-Touchdown PCR and 2) High Annealing Temperature RAPD. Forty three ISSR primers were used in the first approach and 391 loci were detected altogether. All loci generated by this approach were found to be monomorphic across all accessions. Forty one RAPD primers were used in the second approach and 195 loci were detected. One locus was found to be polymorphic that contributed only 0.51% of all banding loci and thus considered of very low variation. It is concluded that these two marker approaches could not detect genetic variation among the A. paniculata accessions subjected for this study. | |
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