ISHS Acta Horticulturae 786: International Workshop on Medicinal and Aromatic Plants
CHARACTERIZATION OF DURIO ZIBETHINUS BY MOLECULAR MARKER AND SOLUBLE POLYSACCHARIDE IN FRUIT RINDS |
| Authors: | O. Nuchuchua, W. Chaipornpokin, R. Maktrirat, D. Phummiratch, S. Pongsamart, S. Sukrong |
| Keywords: | pharmaceutical excipient, antibacterial agent, molecular taxonomy, RAPD analysis, matK gene |
| Abstract:
The properties of polysaccharide gel (PG) extract from fruit rinds of Durio zibethinus Murr. have been studied as a pharmaceutical excipient and antibacterial agent. Bactericidal potency of PG from fruit rinds of certain durian cultivars from Chumphon Province, 'Chani' and 'Puen Muang' (native cultivar) has been found higher than that of 'Mon Thong'. The combination of molecular markers, matK gene sequences and RAPD, and the analysis of PG component in the fruit-rinds was used in the present study to assess the differences of these durian cultivars. The matK gene sequences among durian cultivars were 1,509 bp in length. The nucleotide substitutions presented at position 275 of matK gene of 'Puen Muang' cultivars were either adenosine or cytosine. The cytosine substitutions were also found in 'Mon Thong' and 'Chani'. RAPD patterns of polymorphic bands of certain durian cultivars were elucidated. The total yield of PG in fruit-rinds of 'Mon Thong', 'Chani' and 'Puen Muang' cultivars was 9.287+1.279%, 7.774+1.173% and 7.448+0.925%, whereas the galacturonic acid content in PG was 66.097±10.197%, 54.351±9.459% and 64.330±6.674%, respectively. The total yield and the galacturonic acid content of PG in fruit-rinds of 'Chani' were lower than that of 'Mon Thong' cultivars. The RAPD analysis could be applied to characterize these durian cultivars. | |
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