ISHS Acta Horticulturae 781: XX International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
DETECTION, DISTRIBUTION AND GENETIC DIVERSITIES OF APPLE STEM PITTING VIRUS AND APPLE STEM GROOVING VIRUS IN THE CZECH REPUBLIC |
| Author: | J.K. Kundu |
| Keywords: | ASPV, ASGV, duplex-RT-PCR |
| Abstract:
Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) are well distributed pome fruit tree viruses in Czech orchards and occurs either as single or mixed infections. A reliable and easy to use One-step-(duplex)-RT-PCR tool has been developed for the routine detection of ASPV and ASGV. The virus detection again confirms by sequence data of the viruses derived from PCR amplicons of the 3-terminal part of genome ASPV and ASGV. The nucleotide sequence of 3’ter ASPV genome (‘nt’ position 8992−9220) of tested Czech isolates has shown highly variable (identity range 80−100%). However, deduced amino acid sequences of C-ter coat protein gene have shown that some of these isolates are closely related (identity 100%). The phylogenetic analysis also confirms these results. The analysis of nucleotide and deduced amino acid sequences of 3’ter ASGV genome (‘nt’ position 6060−6481), and phylogenetic analysis of tested isolates has shown high homology among the Czech isolates of ASGV. The sequence identity has ranged ‘nt’ >94−100% and ‘aa’ >96-100%. | |
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