ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes
CHANGES IN ISOPEROXIDASES DURING DIFFERENTIATION OF CULTURED TOBACCO EPIDERMAL LAYERS |
| Authors: | Th. Gaspar, M. Tran Thanh Van, T.A. Thorpe |
| Abstract:
Epidermal explants from floral branches of tobacco plants when grown under in vitro culture conditions form de novo floral or vegetative buds, roots or callus. Changes in soluble isoperoxidases were followed electrophoretically in relation to these varying morphognetic pathways. In each of them, the number of bands increases on both the anodic and cathodic sides with time in culture. Compared to each other the four programmes are different in their peroxidase zymograms, mainly through varying kinetics in the development of activity of the isoenzymes. The curves of total specific peroxidase activity do not necessarily reflect these variations. Changes observed during root and vegetative bud formation agree with previously published data. | |
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