ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes
MAINTAINED CULTURE OF MULTIPLE PLANTLETS FROM CARNATION SHOOT TIPS |
| Authors: | S. Jelaska, R. Sutina |
| Abstract:
Shoot tips (0.2–0.5 mm) of carnation (Dianthus caryophyllus L. ‘William Sim’) consisting of apical dome plus two pairs of leaf primordia were cultivated on Murashige-Skoog agar medium containing 3% sucrose or 4% glucose and combinations of varying levels of IAA, NAA and kinetin. The combination of 0.02 mg/l NAA and 2 mg/l kinetin was best for formation of multiple adventive shoots. On this medium, cultures were composed of many shoots and insignificantly little callus tissue. Inoculi from 5–10 shoots, transferred to fresh medium (of the same composition) every 20 days have continued to form new adventive shoots unchangeably for nearly two years now. The initial plant material showed precipitation lines in agar gel double diffusion test with the antiserum against Carnation mottle virus. Adventive shoots, formed in culture, have been tested in the various passages with the same test method and also with the experiment by reaction on Chenopodium amaranticolor. They have always shown negative reaction to CarMV. | |
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