ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes
PROPAGATION OF HOOP PINE (ARAUCARIA CUNNINGHAMII AIT.) BY ORGAN CULTURE |
| Authors: | R.J. Haines, R.A. de Fossard |
| Abstract:
The specialized physiological characteristics of the Araucariaceae severely limit the extent to which vegetative propagation by cuttings and by most methods of grafting can be used in propagation of species in this family. This paper describes the successful propagation of hoop pine by in vitro culture of the concealed bud traces present in the axils of leaves on the orthotropic shoot. When small stem segments containing bud traces were excised and explanted onto a medium containing mineral nutrients, sucrose, and a number of vitamins, bud traces developed into small orthotropic shoots. On subsequent excision and transfer of these shoots to the same medium with a range of IBA concentrations added, roots developed on a number of the explants. The possible use of this method of propagation in reafforestation programmes is discussed. | |
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