ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes
THE INITIATION, ROOTING AND ESTABLISHMENT OF CORTICAL BUDS IN CAULIFLOWER |
| Authors: | D.S. Trimboli, N. Prakash, R.A. de Fossard |
| Abstract:
Explants from the inflorescence, node and internode of cauliflower were planted on 81 media of the Broad Spectrum experiment (de Fossard, 1976) and exposed to 12 h light/12 h dark at 25°C; pieces of inflorescence were also placed in liquid medium and shaken during the incubation period. Buds grew from inflorescence cultures in both liquid and agar media, and an agar medium with a high concentration of minerals, cytokinins, growth factors and sucrose, and medium concentration of auxins (i.e. Broad Spectrum code, HMHH) was particularly conducive to bud initiation, development and multiplication. Both internodal and nodal explants produced buds from the cortex; buds arose first from the distal end of the explant and later from the proximal end. Development of buds was preceded by the formation of small callus on the cut surface of the cortex and phloem. The shoots which developed from the buds from both inflorescences and cortex were excised and formed roots on a cytokinin-free medium with 10uM IBA (as the sole source of auxin) in the presence of high concentrations of minerals, growth factors and sucrose (i.e. Broad Spectrum code, HHIBAZH). These rooted plants were easily established in soil. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
