ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes
PLANTLET FORMATION FROM EXCISED BULB SCALE SEGMENTS OF NERINE |
| Authors: | R.L.M. Pierik, B.J. Ippel |
| DOI: | 10.17660/ActaHortic.1977.78.25 |
| Abstract:
Excised bulb scale segments of Nerine bowdenii W. Watts. cv. Van Roon produced bulblets which formed roots and leaves after subculture on a fresh medium. Double scales connected by part of the basal plate were grown with basal ends up on a modified Murashige and Skoog medium with 1 mg/1 IBA. Cultures were kept at 25°C in continuous fluorescent light. Special attention was paid to factors affecting the formation and growth of bulblets. The most essential requirement for the formation of bulblets was the presence of part of the basal plate. Bulblet formation was increased by increasing the width and/or the length of the explants. The optimum temperature was 21 to 25°C. The formation and the growth of bulblets were promoted by 0.1–1 mg/1 IBA. Rooting, bulbing and leaf development of subcultured bulblets were much better when explant culture took place in darkness instead of in continuous light. Plantlet formation starting with excised double scales could also be obtained from Nerine sarniensis and Nerine hybrids. | |
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