ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes
SOME FACTORS AFFECTING MULTIPLE BUD FORMATION OF STRAWBERRY (X FRAGARIA ANANASSA DUCHESNE) IN VITRO |
| Authors: | E.C.M. Lee, R.A. de Fossard |
| Abstract:
Very rapid multiplication of strawberry can be achieved using a Broad Spectrum combination of medium concentration of minerals, growth factors and sucrose, with 1.56μM IAA and 50μM kinetin. The cultures are incubated in the dark at 25°C for the multiplication phase and are then transferred to 12h light/12h dark incubation at 25°C for development of leaves and roots. The results of omission type experiments with various constituents of the medium are described, including experiments with auxins, cytokinins and different photoperiods. | |
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