ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes
MORPHOGENESIS AND PLANT REGENERATION FROM CULTURED ORGAN FRAGMENTS OF CYCLAMEN PERSICUM |
| Author: | T. Geier |
| Abstract:
Microbial contamination which is a main obstacle in successful cultivation of plants from Cyclamen tuber tissue explants can be substantially reduced without impairing growth and morphogenesis by precultivation in the presence of the antibiotic Achromycin and subsequent transfer of the explants to a medium without Achromycin. A high degree of balanced development of roots and buds from the same explants is induced by combinations of kinetin and IAA (indoleacetic acid). Well rooted explants with green leaves can be transferred to soil and grown to mature plants. Explants from other plant parts show less morphogenetic potential as compared to tuber tissue. However, regeneration of complete plants can also be achieved starting with somatic anther tissue and fragments of leaf blades. In this case callus is induced in the presence of BA (6-benzylaminopurine) and IAA which gives rise to a variety of leaf-like structures and some normally developed buds. In a second step complete plants are obtained by rooting these buds in the presence of NAA (naphtaleneacetic acid). | |
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