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ISHS Acta Horticulturae 78: Symposium on Tissue Culture for Horticultural Purposes

USE OF MICROPROPAGATION AND TISSUE CULTURE TO INVESTIGATE GENETIC DISORDERS IN ALMOND CULTIVARS

Authors:   D.E. Kester, L. Tabachnik, J. Negueroles
Abstract:
Noninfectious bud-failure (BF) in almond is a "genetic disorder" characterized by heat sensitivity of separate propagation-clones within certain cultivars. A correlation between the in vivo characteristics of separate propagation-clones and their in vitro behavior in tissue culture is shown. BF callus-clones show enhanced proliferation in high nitrogen media at 25°C and depression at 35°C. Differential response between BF and "normal" callus-clones may require growing in low nitrogen medium. Relationship to clonal nomenclature is discussed.

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