ISHS Acta Horticulturae 773: XXVII International Horticultural Congress - IHC2006: International Symposium on Citrus and Other Tropical and Subtropical Fruit Crops
RAPD PROFILING OF FOUR NATIVE CITRUS SPECIES IN TAIWAN |
| Authors: | S.Y. Lin, I.Z. Chen |
| Keywords: | Citrus aurantium, Citrus depressa, Citrus tachibana, Citrus taiwanica, DNA markers |
| Abstract:
Genotypes of 4 native Citrus spp. (Citrus tachibana, C. depressa, C. taiwanica, and C. aurantium) of Taiwan have been studied by using Random Amplified Poly¬morphic DNA (RAPD) markers. We also estimated the relations between the 4 native Citrus species and the popular cultivated Citrus species. The analysis was conducted by orange- and mandarin group. Fifty one arbitrary 10-mer primers generated 277 randomly amplified DNA fragments in orange group, and 13 primers generated 189 randomly amplified DNA fragments in mandarin group; these were scored for the analysis. The 4 native Citrus species have been successfully distinguished from each other and other cultivated species by the RAPD analysis. The RAPD profiles of wild samples of C. depressa and C. taiwanica were slightly different from the specimen conserving in CAES, which showed the diversity possibly resulting from the environmental stress. Further work is needed in establishing the easy-identification method for wild samples of native Citrus species by RAPD markers. | |
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