ISHS Acta Horticulturae 769: XXVII International Horticultural Congress - IHC2006: International Symposium on Asian Plants with Unique Horticultural Potential
QUANTITATIVE ANALYSIS OF FATTY ACIDS IN LEAVES OF LIGUSTRUM QUIHOUI |
| Authors: | Zhi-hui Li, Han-zhou Sun, Li-qun Huang, Li-xin He, Fang-de Lv |
| Keywords: | Ligustrum quihoui, internal standard, fatty acid, quantitative analysis |
| Abstract:
The cold hardiness of a landscape plant is an important factor in regulating its introduction and cultivation. Thankfully, unsaturated fatty acids in a plant can be used as a determinant of its cold hardiness. The quantities of fatty acids in the leaves of Ligustrum quihoui were analyzed using CHCl3-CH3OH mixed solvent distilling, pentadecanoic acid internal standard, KOH-CH3OH-BF3 esterify, silica gel discoloration, and finally, gas chromatography. The results concluded that myristic acid was not detectable, but the quantities of palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, and eleosetearic acid could be found. The relative response factor for each of the fatty acids was 1.000, 0.8886, 0.9998, 0.8684, 0.7551 and 0.5334, respectively. The linear correlative coefficient of each of the relative response factors was 0.9998, 0.9984, 0.9993, 0.9979, 0.9968 and 0.9947. The relative standard deviations were 2.52, 3.98, 3.18, 2.71, 2.39 and 1.62%. The recovery rates were 96.97, 107.52, 88.67, 91.32, 94.22 and 92.48%. All the linear correlative coefficients of the relative response factors of various fatty acids were over 0.99. The relative standard deviations were less than 5.0%. The recovery rates ranged from 88.67 to 107.52%. This data indicates that the quantitative analysis of trace elements can be conducted using this method. | |
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