ISHS Acta Horticulturae 766: XXVII International Horticultural Congress - IHC2006: International Symposium on Ornamentals, Now!
MICROPROPAGATION OF ARDISIA PUSILLA AND ARDISIA JAPONICA IN VITRO |
| Authors: | D.H. Goo, O.K. Kwon, Y.R. Lee, E.J. Huh |
| Keywords: | tissue culture, growth regulators |
| Abstract:
Explants of Ardisia pusilla A. DC. and Ardisia japonica (Thunb.) Blume were established in vitro from greenhouse grown plants in primary cultures. The primary cultures tested lateral buds, shoot tips, and rhizomes as sources for micropropagation materials as influenced by plant growth regulators benzyl adenine (BA), indoleacetic acid (IAA) and naphthalene acetic acid (NAA) and the number of nodes per explant. In A. pusilla, lateral buds were the best primary culture source and 59% of explants produced shoots in 17 days. Shoot tips produced shoots in 30 days from 6% of explants. The results for A. japonica were similar to A. pusilla for primary culture; 27 days and 15%, respectively, for shoot formation from the lateral buds and 42 days and 12% from the shoot tip explants. Due to bacterial contamination, explants from rhizomes in both species failed to regenerate. Only one shoot in both species was produced from the primary cultures. In multiplication subcultures, BA at 0 to 5.0 mg L-1 in combination with IAA at 0.1 to 5.0 mg L-1 was tested. Effective concentrations for A. japonica were 1.0 mg L-1 BA and 0.1 mg L-1 IAA to yield 17 days for shoot formation, 100% sprouting, and 4.8 shoots. Effective concentrations for A. pusilla for shoot formation were 0.5 mg L-1 BA and 0.10 mg L-1 IAA to yield 62% sprouting in 18 days, forming only one shoot produced. A. pusilla explants required at least two nodes for multiplication during subculture. | |
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