ISHS Acta Horticulturae 764: XXVII International Horticultural Congress - IHC2006: International Symposium on Plant Biotechnology: From Bench to Commercialization
EFFECT OF COLD TREATMENT TIME ON STEM-DISC CULTURE OF GARLIC (ALLIUM SATIVUM L.) |
| Authors: | Z. Wu, Y.J. Long, Q. Zhang, P.F. Li |
| Keywords: | cold treatment, garlic, micro-bulbs, propagation coefficient, stem-disc |
| Abstract:
In garlic tissue culture, cytokinin generally needs to be added in medium to increase seedling propagation coefficient. However, in this study, appropriate cold treatment at 4°C was also found to have the same effect on garlic bulb propagation. Using Chinese local garlic variety (‘Sanyuehuang’) as the material and LS as the medium, cold treatment at 4°C respectively for 0, 2, 4, 6, 8 and 10 weeks, we studied the propagation and bulbs development of garlic in stem-disc culture. The results showed that cold treatment could enhance the propagation coefficient of garlic and accelerate garlic bulb development. The best effects were found in cold treatment for 6 and 4 weeks, while the propagation coefficients were high, up to 7.34 and 3.85, respectively. At the same time, the ratio of micro-bulbs development reached 89.6% and 54.7%, rooting number was 2.8 and 4.9 with the average weight of bulb 70~80 mg. The germination ratios of micro-bulbs were correlative to their own weights, and the germination ratios would be 100% when bulb weights were over 50 mg. | |
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