ISHS Acta Horticulturae 764: XXVII International Horticultural Congress - IHC2006: International Symposium on Plant Biotechnology: From Bench to Commercialization
SHOOT REGENERATION AND GENETIC TRANSFORMATION OF REGENERATIVE CALLUS CULTURE IN DIANTHUS HYBRIDA 'TELSTAR SCARLET' |
| Authors: | C. Nontaswatsri, K. Uchiyama, S. Fukai |
| Keywords: | Agrobacterium-mediated genetic transformation, Dianthus hybrida ¿Telstar Scarlet¿, hormones |
| Abstract:
Regenerative callus was established and was successfully applied to Agrobacterium-mediated genetic transformation. The callus was isolated from the base of adventitious shoots on cotyledon explants of Dianthus hybrida cv. ‘Telstar Scarlet’ cultured on MS media supplemented with 1 mg L-1 TDZ and 0.1 mg L-1 NAA. This callus culture was successfully applied to Agrobacterium-mediated genetic transformation. Transformed shoots were obtained from inoculated calli selected on MS medium containing 1.0 mg L-1 TDZ, 0.1 mg L-1 NAA, 20 g L-1 sucrose, 2 g L-1 gellan gum and 20–80 mg L-1 G-418 within 20 weeks from inoculation. All transformants grew, flowered normally and produced seeds when crossed with other Dianthus species. | |
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