ISHS Acta Horticulturae 764: XXVII International Horticultural Congress - IHC2006: International Symposium on Plant Biotechnology: From Bench to Commercialization
REDUCED LEAF SENESCENCE IN CHRYSANTHEMUM TRANSFORMED WITH MUTATED ETHYLENE RECEPTOR GENES |
| Authors: | S. Satoh, T. Narumi, M. Watanabe, R. Aida, A. Ohmiya |
| Keywords: | Dendranthema grandiflorum, transformation |
| Abstract:
Leaves of cut chrysanthemum of some cultivars frequently become yellow, sometimes prior to the onset of flower senescence. The leaf yellowing is induced by the action of exogenous ethylene. We generated chrysanthemum plants transformed with a mutated ethylene receptor gene (mDG-ERS1(etr1–4)), derived from a chrysanthemum ethylene receptor (DG-ERS1) cDNA. Cultured plantlets of the transformants showed reduced sensitivity to ethylene resulting in reduced leaf yellowing after exposure to exogenous ethylene. When shoots were detached from soil-grown plants natural senescence was inhibited in the shoots detached from soil-grown transformants as compared with those of the non-transformed control. These results suggest that the mutated ethylene receptor gene mDG-ERS1(etr1–4) can be used to generate transgenic chrysanthemums with leaves green for a longer time and thus having a longer vase life. | |
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