ISHS Acta Horticulturae 763: XXVII International Horticultural Congress - IHC2006: International Symposium on Structural and Functional Genomics of Horticultural Plants
ANALYSIS OF CMS AND ITS RESTORATION IN CHILI PEPPER |
| Authors: | G. Gulyas, K. Pakozdi, J.S. Lee, Y. Hirata |
| Keywords: | genetic distance, recombination ratio, restorer marker |
| Abstract:
The practical importance of the CMS system in chili pepper breeding is highly dependent on the presence of a restorer of fertility (Rf or Fr) gene and stable response in various environments. In the present study, the mechanism by which the restorer lines act on the suppression of the CMS phenomenon was further characterized by analyzing F1-F4 generations obtained from the crossing of CMS and restorer lines. The segregation pattern of the F2 generation indicated a mono-factorial inheritance associated with the restorer gene. This was also confirmed by using the subsequent (F3 and F4) generations. In addition, we examined the restorer marker and its genetic distance from the Rf gene to consider the possibility of application to breeding programs, with emphasis placed on the F3 and F4 generations. The recombination ratio in the F2 generation revealed quite far distance (20 cM), however, in the F3 and F4 generations of a second cross the distance was lower, 6.5 cM and 5.9 cM, respectively. It appears therefore that the genetic distance between the marker and the restorer gene is greatly affected by the genetic background. | |
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