DEVELOPMENT OF MOLECULAR MARKERS LINKED TO THE TRAITS OF NON-ACID/ ACID FRUIT OF PEACH

Authors:	Jun Wu, Huairui Shu, Kaichun Zhang, Lijie Jiang
Keywords:	Prunus persica (L.) Batsch, AFLP, SCAR
Abstract: AFLP and bulked segregant analysis were used to screen markers linked to the non-acid/acid trait of peach fruit controlled by D/d gene. Of 64 pairs of primer combinations, twelve amplified polymorphic DNA fragments between non-acid bulk and acid bulk. Only two specific fragments were linked to acid trait (recessive d location), after investigating the progenies. Specific fragments TA-CTC₁₄₀ and AT-CTA₂₀₀ were derived from primers E-TA/M-CTC and E-AT/M-CTA. The linkage distances to D/d gene were 1.47cM and 2.99cM, respectively. Specific primers BFPA₁/A₂ and BFPB1/B2 were designed according to the sequences of two specific fragments. With primers BFPB₁/B₂, genomic DNA amplified the polymorphic band AT-CTA₁₅₈ in acid bulks and individuals. The AFLP marker AT-CTA₂₀₀ was converted into a SCAR marker. The linkage distance of marker AT-CTA₁₅₈ to d gene was the same as that of the original AFLP marker. However, the AFLP marker TA-CTC₁₄₀ was not converted into a SCAR marker. Polymorphism disappeared in both parents and progenies when amplified with primer pair A₁/A₂.
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