ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
A NEW SHORT-CATKIN SPORT IN CHESTNUT (CASTANEA MOLLISSIMA BL.) |
| Authors: | Y. Feng, Y. Xu, Y. Shen, L. Qin |
| Keywords: | AFLP, inflorescences, staminate flowers |
| Abstract:
A chestnut tree with a new short-catkin sport, with an average catkin length of 2.18 cm, was found in an orchard in Beijing. This sport was successfully propagated vegetatively by grafting. The objective of this project was to test for molecular differences between this sport and normal chestnuts using amplified fragment length polymorphism (AFLP). We obtained 5,129 clear bands of 53 polymorphic loci amplified by 72 selective primer pairs using AFLP from DNA extracted from leaves of the short-catkin sport and that extracted from leaves of chestnut with normal length catkins. The polymorphic rate was 2.05% and Nei’s association coefficient was 0.9897. The amplified band range of the polymorphic fragments ranged from 300 to 900bp. The results indicated that the short-catkin sport differed from the normal chestnut at the molecular-level. We also observed that specific segments could be used as markers for the short catkin genetic trait. | |
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