ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
MORPHOLOGICAL TRAITS IN RIBES NIGRUM POLYPLOIDS |
| Authors: | A. Sasnauskas, G. Stanienė, D. Gelvonauskienė, T. Siksnianas, V. Stanys, C. Bobinas, R. Rugienius, D. Baniulis |
| Keywords: | polyploidy, black currant, in vitro, morphological traits, amphidiploid |
| Abstract:
The occurrence of natural polyploids in currants and gooseberries Ribes L. are unknown. The evolution of currants and gooseberries has occurred at the diploid level. Artificially induced tetraploid Ribes nigrum cultivars are unknown. Only a few amphidiploid R. nigrum × R. uva-crispa cultivars have commercial value. In this study, we investigated currant polyploidisation in vitro using isolated embryos. Under the influence of colchicine and oryzaline, triploid, tetraploid and aneuploid plants regenerated from isolated embryos together with diploid plants. With orysalin, a frequency of 15.6 to 19.3 % embryo regeneration was observed. Morphometric and morphological features, including leaf area, berry and inflorescence characteristics, one-year shoot length, resistance to fungal diseases, pollen germination rate were evaluated. Tetraploid regenerated plants were distinguished by morphological leaf and shoot features, berry size, seed number and weight. Resistance to leaf diseases was similar in tetraploid and diploid plants. | |
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