ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
MICROPROPAGATION AND CRYOPRESERVATION OF IN VITRO SHOOT TIPS OF 'SUIZHONGHONG' PAPAYA |
| Authors: | Z. Jiwu, Y. Ganjun, Z. Qiuming |
| Keywords: | Carica papaya L., vitrification |
| Abstract:
Papaya (Carica papaya L.) is an important fruit species and is widely cultivated in many tropical and subtropical countries. The conventional methods of maintaining papaya germplasm under nursery and orchard conditions require extensive space and labor. It is very difficult to conserve papaya germplasm by conventional methods. In this study shoot tips from papaya were multiplied in vitro and cryopreserved by vitrification. Micropropagation was improved by testing growth regulator concentrations. Shoots grown on MS medium with 0.5 mg/L BA, 0.1 mg/L NAA and 1.0 mg/L GA3 were significantly better than on other treatments. Optimized vitrification procedures indicated that for papaya the best protocol was with shoot tips (2-3 mm) precultured for 3 days at 25°CV on MS medium with 5% DMSO and 5% sucrose. Shoot tips (1.5~2.5 mm) were pretreated in 60% PVS2 for 50 min at room temperature, then they were completely dehydrated with 100% PVS2 for 30 min at 0°C. After changing the solution with fresh PVS2 the materials were plunged directly into liquid nitrogen and stored for at least 24 hours. Shoot tips were rapidly warmed in a 40°Cwater bath, and washed twice with 1.2 M sucrose solution and transferred to the optimized medium. Shoot tip regrowth was 52.6%. The shoots developed normally, rooted and survived transplantation. | |
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