ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
MICROSATELLITE MARKERS IN POMEGRANATE |
| Authors: | M. Koohi-Dehkordi, B.E. Sayed-Tabatabaei, A. Yamchi, A. Danesh-Shahraki |
| Keywords: | microsatellite, Punica granatum, Simple Sequence Repeat isolation, SSR enrichment |
| Abstract:
Simple sequence repeats (SSR) or microsatellite markers are an excellent tool for cultivar identification and for the evaluation of genetic distances among organisms. Pomegranate (Punica granatum L.) cultivars have been characterized mainly by common name and morphological traits. The objectives of the present study were to construct microsatellite-enriched libraries from pomegranate and to identify microsatellite-containing sequences. Two genomic libraries enriched for AG and GT repeats were constructed from 'Malase Yazd'. The enrichment was efficient and was 51% in the GA library and 47 % in the GT library. Primer pairs were designed for 15 microsatellite-containing sequences. Five out of 15 primer pairs amplified the expected size fragment and were polymorphic in 29 pomegranate individuals. We will use these SSRs to analyze genetic relationships among pomegranate accessions and for cultivar identification. | |
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