ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
SCREENING OF STRAWBERRIES WITH THE RED STELE (PHYTOPHTHORA FRAGARIAE) RESISTANCE GENE RPF1 USING SEQUENCE SPECIFIC DNA MARKERS |
| Authors: | A. Sasnauskas, R. Rugienius, D. Gelvonauskiené, I. Zalunskaitė, G. Stanienė, T. Siksnianas, V. Stanys, C. Bobinas |
| Keywords: | Fragaria, sequence specific DNA markers, PCR, cultivars |
| Abstract:
Many strawberry (Fragaria × ananassa Duchesne ex Rozier) cultivars are susceptible to the red stele pathogen Phytophthora fragariae var. fragariae Hickman. Red stele is a serious problem for strawberry producers in northern Europe. The disease is most destructive in heavy clay soils that are saturated with water during cool weather when the pathogen is most active. Elimination or attenuation of the symptoms and losses caused by the pathogen can be only achieved by introduction of resistant cultivars because of the lack of registered environmentally safe chemicals against Phytophthora spp. Screening strawberries for red stele resistance utilizing DNA markers is an efficient strategy. Strawberry Rpf1 gene confers resistance to 16 P. fragariae races. Using polymorphic DNA oligonucleotide primers and PCR we have amplified strawberry DNA fragments associated with Rpf1 gene. The DNA bands were cloned and sequenced. Primers for screening for red stele sensitivity and lack of Rpf1 gene were designed according the sequencing results. PCR-SSP 11 strawberry varieties and seedlings of four crosses were screened. | |
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