ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
ANALYSIS OF AMERICAN BLACK WALNUT GERMPLASM USING RAPD MARKERS |
| Authors: | X. Shen, J. Zhang, L. Wang, X.L. Li, J.X. Zhang |
| Keywords: | Juglans nigra, idioplasmic resource, morphological characters, randomly amplified polymorphic DNA |
| Abstract:
American black walnut (Juglans nigra L.) germplasm was imported from the United States in the early 1990s. Using morphological characters, three year old plants were classified into three groups designated Type I, II and III. Type I were fast-growing seedlings with small leaves; Type II were fast-growing seedlings with large leaves; type III were slow growing seedlings with large leaves. The common walnut, Juglans regia L. was designated as an out-group, Type IV. Twenty one randomly amplified polymorphic DNA (RAPD) decamer primers were chosen to analyze the seedlings. The primers S10 and S8 produced bands 600 bp and 560 bp, respectively that were associated with fast growth. The primer S15 produced a band at 520 bp that was associated with slow growth. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis was able to reveal significant variations among the three groups at the molecular level. | |
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