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ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops

AFLP ANALYSIS OF FOUR WILD MALUS MILL.

Authors:   Q. Guo, Y. Yu, Q. He, X. Li, G. Liang
Keywords:   apple, wild species, amplified fragment length polymorphism
Abstract:
Amplified fragment length polymorphism (AFLP) markers were used to assess polymorphism in wild Malus. Three primer pairs detected a total of 228 AFLP fragments in 27 accessions of mostly Malus hupehensis Rehd., M. baccata Borkh, M. toringoides (Rehd.) Hughes and M. sieboldii Rehd. Up to 216 fragments (94.7%) were polymorphic. UPGMA analysis identified two clades: an M. toringoides clade of mixed ploidy levels and a mixed clade containing the remaining species. This study supports the close relationship between M. sieboldii, M. baccata and M. hupehensis and agrees with previous morphological characterization, flavonoid and isozymes analyses.

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