ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
GENETIC FINGERPRINTING OF THE IRISH APPLE HERITAGE COLLECTION |
| Authors: | S. Mac an tSaoir, F. Ward, C. Fleming, B. Moreland |
| Keywords: | microsatellites, Omar, Chris, M2, TCC5, GACA4 |
| Abstract:
A collection of Irish apple (Malus × domestica) trees has been established in the walled garden of Loughgall Country Park at Armagh, Ireland. The collection was established by the Armagh Orchard Trust under the management of the local apple research unit. The trees were collected from four sources – Seed Savers Conservation, Co Clare, University College Dublin Collection, Ardress House Collection Co Armagh, and from members of the general public. As the collection grew, the trees were phenotypically described. Since most of the trees had local names, there was a high risk of duplication, either the same trees with different local names or a commercial foreign tree with a local name. As the collection was phenotypically examined, it became evident that there were many potential duplicates. It was decided to use microsatellites to characterise the collection. Five primers were selected and used to generate polymerase chain reaction (PCR) products from individual DNA samples taken from the collection. These products were separated on high resolution gels, coded and subjected to bootstrap analysis and near neighbour analysis. Significant relationships were found amongst the collection but the number of microsatellites used was too small to distinguish between all the cultivars tested. | |
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