ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
IDENTIFICATION OF S-ALLELES IN 40 APPLE (MALUS X DOMESTICA BORKH) CULTIVARS BY ALLELE-SPECIFIC PCR AMPLIFICATION |
| Authors: | A. Ershadi, A. Talaii |
| Keywords: | apple, S-allele, PCR, sequencing |
| Abstract:
The S–alleles of 40 apple cultivars including 12 old cultivars previously genotyped by hand pollination were identified based on allele – specific polymerase chain reaction (PCR) amplification using primers designed for S2 , S3 , S4 , S5 , S7 , S9 , S10 , S24, S26 , S27 , Sf , Sd and Se alleles. Using the given method all S – alleles in three triploid cvs. and 21 diploid cvs. were identified. However, only two S-alleles in six triploid cvs. and one S-allele in ten diploid cvs. were detected. S22 and S23 alleles formerly identified in Alkmene and Delbard Jubilee cvs by means of stylar ribonuclease analysis were amplified with S27 allele primers. Sequencing the corresponding PCR products showed that S22, S23 and S27 alleles are the same. Also S18 allele which distinguished in Menzauer Jagerapfel based on isoenzyme analysis amplified with Sd specific primer and their corresponding PCR product were 100% identical. Although allele–specific PCR amplification was not capable to identify all S–alleles in studied apple cultivars, but it provides a rapid and useful approach for determining the S-genotype of apple cultivars. | |
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