ISHS Acta Horticulturae 758: X International Symposium on the Processing Tomato
STANDARDIZATION OF A RAPID SPECTROPHOTOMETRIC METHOD FOR LYCOPENE ANALYSIS |
| Authors: | G. Anthon, D.M. Barrett |
| Keywords: | tomato, lycopene, analysis, spectrophotometric |
| Abstract:
Health professionals and consumers are becoming increasingly aware of the benefits of lycopene consumption; tomatoes provide the greatest source of this important phytonutrient. Our laboratory has developed a standard method for the extraction and determination of lycopene in tomato products. This is a sensitive, rapid and reproducible assay utilizing a mixture of hexane/ethanol/acetone for extraction. Following incubation for 1 hour, the hexane layer is removed using a Drummond pipettor and lycopene is determined by measuring absorbance at 503 nm. This method will be useful for breeding programs and processors who are interested in evaluation of a large number of samples in a rapid fashion. In this manuscript we describe the method and how it was validated. | |
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