ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 758: X International Symposium on the Processing Tomato

STANDARDIZATION OF A RAPID SPECTROPHOTOMETRIC METHOD FOR LYCOPENE ANALYSIS

Authors:   G. Anthon, D.M. Barrett
Keywords:   tomato, lycopene, analysis, spectrophotometric
DOI:   10.17660/ActaHortic.2007.758.12
Abstract:
Health professionals and consumers are becoming increasingly aware of the benefits of lycopene consumption; tomatoes provide the greatest source of this important phytonutrient. Our laboratory has developed a standard method for the extraction and determination of lycopene in tomato products. This is a sensitive, rapid and reproducible assay utilizing a mixture of hexane/ethanol/acetone for extraction. Following incubation for 1 hour, the hexane layer is removed using a Drummond pipettor and lycopene is determined by measuring absorbance at 503 nm. This method will be useful for breeding programs and processors who are interested in evaluation of a large number of samples in a rapid fashion. In this manuscript we describe the method and how it was validated.

Download Adobe Acrobat Reader (free software to read PDF files)

758_11     758     758_13

URL www.actahort.org      Hosted by KU Leuven      © ISHS