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ISHS Acta Horticulturae 757: I International Symposium on Breadfruit Research and Development

STUDIES ON IN VITRO CULTURE OF BREADFRUIT CULTIVARS IN THE PACIFIC

Authors:   V.S. Tuia, M.B. Taylor, D. Ragone
Keywords:   micropropagation, Centre for Pacific Crops and Trees, explants, budding, tissue culture
Abstract:
This paper outlines an in vitro protocol for breadfruit, Artocarpus altilis. Juvenile explants obtained from trimmed root suckers potted in the screenhouse proved to be the optimum source of material for in vitro establishment with a mean survival rate of 78%. In contrast, a 64% mean survival rate was obtained with explants derived directly from field-grown, trimmed root suckers. Explants less than 10 mm rooted in 1-2 months, compared to explants greater than 10 mm, where roots developed after 3-4 months in culture. The immersion of explants in 100 mg/L citric acid during treatment minimized browning. Woody plant medium (WPM) was the optimal medium in terms of survival, root production and shoot growth, with multiplication enhanced with the addition of 2.5 mg/L of 6-benzylaminopurine (BAP) to the medium.
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