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ISHS Acta Horticulturae 755: International Conference on Quality Management in Supply Chains of Ornamentals

SOMACLONAL VARIATION OF CALADIUM [CALADIUM BICOLOR (AIT.) VENT.] FROM IN VITRO PROPAGATION

Authors:   U. Somkanea, C. Thapsithar, A. Thongpukdee
Keywords:   Caladium, micropropagation, somaclonal variation, variants
Abstract:
Variations in caladium [Caladium bicolor (Ait.) Vent.] ‘Arjarn Pramote’ and ‘Suvarnabhum’ from in vitro micropropagation were observed. Unexpanded leaf segments were cultured on modified Murashige and Skoog medium (MS) supplemented with 2.69 µM 1-naphthalene acetic acid (NAA) and 17.76 µM N6-benzyladenine (BA) for 4 months. From 3 morphological groups – leaf pattern, petiole and leaf colour, regenerated caladium plants were divided into 14 and 5 types for ‘Arjarn Pramote’ and ‘Suvarnabhum’, respectively. Variations were found in 13 types with multiple variants from 2-7 characters in ‘Arjarn Pramote’, while 4 types with multiple variants from 2 and 4 characters occurred in ‘Suvarnabhum’. The occurrence of variants was 66.35 and 56.00% in regenerated plants from ‘Arjarn Pramote’ and ‘Suvarnabhum’, respectively. For ‘Arjarn Pramote’, leaf color variants were observed at the highest frequency of 64.42% while leaf pattern variants and petiole variants were found at 51.92 and 22.11%, respectively. Similarly, 35.00% leaf color variants, 34.00% leaf pattern variants and 0.00% petiole variants were found in ‘Suvarnabhum’.

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