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ISHS Acta Horticulturae 755: International Conference on Quality Management in Supply Chains of Ornamentals

BREEDING AND MICROPROPAGATION OF AGLAONEMA

Authors:   D.M. Yeh, W.J. Yang, F.C. Chang, M.C. Chung, W.L. Chen, H.W. Huang
Keywords:   Aglaonema, flowering, chromosome, genetic relationship, micropropagation
Abstract:
Breeding for Aglaonema (Araceae) cultivars with beautiful foliar variegation and color has long been the prevailing goal. The present works studied on regulation of flowering, chromosome number and genetic relationship to facilitate hybridization. Plants flower within 23 to 48 weeks, depending on the cultivar, following a single spray with 250 ppm gibberellic acid (GA3). RAPD analysis on 61 accessions showed that Aglaonema genotypes are highly diverged and could be clustered into seven groups. Cultivars ‘Curtisii’ and ‘Galaxy’ are diploid (2n = 40), ‘Pride of Sumatra’ and ‘Chalit’s Fantasy’ have 50 and 60 chromosomes, respectively. At least eight dominant alleles each with distinct pattern have been identified. Tissue culture is preferable for rapid multiplication of healthy plants. The inflorescence was an alternative source of explants to reduce endogenous microbial contamination, and the suggested cultural medium was half-strength MS basal medium with 5 to 10 µM Dicamba and 10 µM TDZ for direct shooting. After transferring to a shaded greenhouse, plants under 130 μmol.m-2.s-1 during ex vitro acclimatization had higher dry weight than those under 80 or 200 μmol.m-2.s-1.

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