ISHS Acta Horticulturae 754: International Workshop on Advances in Grapevine and Wine Research
ASSAY OF THE FUNGICIDAL ACTION OF ACETYLSALICYLIC ACID ON BOTRYTIS CINEREA |
| Authors: | A. Moret, M. Nadal, Z. Muņoz |
| Keywords: | ASA, CI50, dematiaceae, fungal inhibition, mycelial growth, regression line |
| Abstract:
Botrytis cinerea is a plant pathogenic fungus of the Dematiaceae family that cause a serious disease in a wide range of hosts including many economically valuable plant species, such as vegetables, ornamental plants, vines and fruit trees. This pathogen reduces productivity and causes considerable losses. The objectives of this study were to evaluate the effect of acetylsalicylic acid (ASA) in vitro on the mycelial growth of B. cinerea and to determine the concentration that would inhibit 50% of its growth (CI50). Five concentrations of ASA (50, 100, 250, 350 and 450 ppm) were assayed and compared to an untreated control. ASA was incorporated into Potato-Dextrose-Agar (PDA) medium at 60°C and poured in 90 mm Petri dishes to yield a total volume of 15 ml/plate. A 5 mm-diameter plug from an actively growing PDA culture was placed fungus-side down in the center of the Petri dishes. Plates were incubated at 25°C for 7 days in the dark. A total of 80 replicates were done for each concentration and colony diameters were measured daily. The regression line and the CI50 were calculated from the mean daily growth of the colonies in each of the concentrations assayed. B. cinerea was found to be sensitive to ASA treatment. The straight-line regression equation was Y= 568.082-9.37465X with r2 = 0.99. From this line, a CI50 of 299.36 ppm was calculated. The use of ASA in field control of fungal infections could reduce levels of environmental pollution and attenuate the problems of resistance caused by continual use of a phytosanitary product over long periods of time. | |
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