ISHS Acta Horticulturae 754: International Workshop on Advances in Grapevine and Wine Research
COMPARISON OF LABORATORY LEAF DISK TECHNIQUE AGAINST TRADITIONAL METHODS IN HOTBED AND FIELD SCREENING PROCEDURES FOR EVALUATION OF GRAPE SEEDLINGS FOR DOWNY MILDEW RESISTANCE |
| Authors: | R. Sotolář, M. Vachůn |
| Keywords: | Downy mildew, interspecific hybrid, resistance, disease, sporulation, Leaf disk technique |
| Abstract:
This research was conducted to compare a laboratory technique leaf disk with hotbed and field evaluations for screening large populations of grape seedlings (Vitis spp.) against downy mildew (Plasmopara viticola Berk. et Curt.) resistance. Seedlings produced by crossing of two hybrid families, in (Vitis amurensis RUPR. × Vitis vinifera L.) × (Franco-American hybrid × Vitis vinifera L.) and antipodal crossing, and Franco- American hybrid × Vitis vinifera L. × (Franco-American hybrid × Vitis vinifera BC) combinations. The cultivars ‘Bianca’, ‘Merzling’, ‘Kunleany’, ‘Hibernal’ and ‘Hibia’ were used. These varieties were rated for sporulation for five levels from 0 to 80 percentages. Sporulation ratings were evaluated at the first and second rating. Significant differences between field evaluations and leaf disk technique was found. Also a highly significant correlation was found between leaf disk technique and hotbed evaluation. The leaf disk procedure appeared to be a good predictor of field resistance and is more practical than the hotbed provocation method for screening large populations. | |
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