ISHS Acta Horticulturae 751: IV International Symposium on Rose Research and Cultivation
EFFECTS OF SEVERAL VARIABLES ON IN VITRO CULTURE OF DAMASK ROSE (ROSA DAMASCENA MILL.) |
| Authors: | M. Khosh-Khui, Z. Jabbarzadeh |
| Keywords: | micropropagation, proliferation, rooting |
| Abstract:
To evaluate the regeneration ability of Damask rose, single-node explants were surface sterilized and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of N6-benzyladenine (BA) or kinetin (Kin) separately or in combination with different concentrations of indole-3-butyric acid (IBA). Combination of 2.5-3 mg L-1 BA and 0.1 mg L-1 IBA was the most suitable treatment for proliferation. In vitro derived shoots were subcultured four times on the fresh medium within a four-week period. Effects of basal salts concentrations, using different auxins in medium or in quick-dip method, using various concentrations of abscisic acid (ABA) in combination with various concentrations of IAA, IBA and NAA, and using various concentrations of sucrose and agar did not produce any roots. The best treatment for rooting of shoots was 2.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) for two weeks in MS medium and then transferring the explants to MS medium without any growth regulator. Plantlets were acclimatized in a soil mixture consisting of peat moss and sand 1:1 (v/v) and successfully transferred to the greenhouse after three weeks. | |
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