ISHS Acta Horticulturae 748: II International Symposium on Acclimatization and Establishment of Micropropagated Plants
SURVIVAL AND GROWTH ON SOIL OF MICROPROPAGATED PITAYA DE MAYO PLANTS |
| Authors: | M.L. Martínez-Cárdenas, R. Vicente-Solano, A. Martínez-Herrera, A. Carmona, G. Varela H. |
| Keywords: | Stenocereus griseus, Cactaceae, propagation, tissue culture, acclimatation |
| Abstract:
Pitaya de mayo is the fruit of a columnar cactus, Stenocereus griseus. Its cultivation and marketing take place principally in the Mixteca region of Oaxaca and The Tehuacan Valley, Puebla. It is a big sweet fruit, harvested in May. There are many producers but the pitaya fruit is produced only in family orchards with an average of 1000 m2 of surface, with 1600 plants ha-1, the mean yield renders 45.33 boxes of 30–35 kg each. The fruit is consumed freshly, as cool drink, ice cream, jelly, jam and spirit drinks. The micropropagation of pitaya will allow to extend the area of the crop and its preservation. Our objective was to micropropagate pitaya de mayo up to the stage of its establishment on soil. Epicotyls from pitaya de mayo seedlings were grown on MS medium alone or with NAA, IAA, NAA-BAP or IAA-BAP (0.1 mg L-1) to induce shoot development, then they were placed on MS to produce roots and then they were transplanted to soil. Measurements of growth, number of areoles and the survival were registered. The MS, MS plus IAA or NAA medium only produced roots, MS plus IAA-BAP or NAA-BAP produced 2–4 shoots/epicotyl at 70%, all of them produced roots on MS medium. On soil, the plants from MS, IAA and AIA-BAP had the best survival and growth, 48 areolas and 4.0 cm in 10 months when they were in a growth chamber (30°C, 16 h light). In the green house, all of them died and ANA and ANA-BAP also. | |
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