ISHS Acta Horticulturae 748: II International Symposium on Acclimatization and Establishment of Micropropagated Plants
CYMBIDIUM: SUCCESSFUL IN VITRO GROWTH AND SUBSEQUENT ACCLIMATIZATION |
| Authors: | J.A. Teixeira da Silva, A. Norikane, M. Tanaka |
| Keywords: | Cymbidium, flow cytometry, RAPDs, somaclonal variation |
| Abstract:
Studies focussing on the capacity to induce protocorm-like bodies (PLBs) or somatic embryogenesis in ornamental, hybrid Cymbidium are still rare. Often in vitro regeneration studies claim no variation based on morphological traits alone, without cytogenetic confirmation. The induction of PLBs and embryogenic callus (EC) by different plant growth regulators (singly-applied, or in combination) were tested. Resulting organs (shoots, adventitious roots, EC, PLBs) were tested for genetic variability and somaclonal variation using flow cytometry and RAPD analysis. Ex vitro Cymbidium plantlet genetic stability was also tested and compared. | |
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