ISHS Acta Horticulturae 748: II International Symposium on Acclimatization and Establishment of Micropropagated Plants
MULTIPLICATION AND ACCLIMATIZATION OF 'NORTON' GRAPEVINE (VITIS AESTIVALIS) |
| Authors: | B.B. Bigger, P.E. Read |
| Abstract:
Commercial propagators of ‘Norton’ (also known as ‘Cynthiana’) often experience difficulty in propagating this cultivar by cuttings, the normally employed method of grapevine multiplication. Therefore, we endeavored to propagate ‘Norton’ by micropropagation. Cultures were readily established in vitro by placing axillary buds taken from 3-year-old greenhouse-grown potted vines on Murashige and Skoog medium supplemented with 4 µM benzyladenine (BA) and 0.5 mg thiamine per liter. When in vitro-derived axillary buds were cultured on various levels of BA and naphthaleneacetic acid (NAA), excellent multiplication was obtained when 4 to 8 µM BA was included in the medium. NAA had little effect on shoot number or number of nodes per shoot. Plantlets were simultaneously acclimated and rooted ex vitro in rehydrated compressed peat pellets (Jiffy-9), with or without treatment with 1000 ppm indolebutyric acid (IBA) as a 5-second dip. Rooting was nearly 100%, regardless of IBA treatment. Over 90% of rooted microcuttings were successfully established in the greenhouse and later transferred to the field. | |
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