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ISHS Acta Horticulturae 748: II International Symposium on Acclimatization and Establishment of Micropropagated Plants

PITAHAYA (HYLOCEREUS UNDATUS) ACCLIMATIZATION: A PEDAGOGICAL MODEL

Authors:   D.P. Orea C., A. Medrano V.
Keywords:   Hylocereus undatus, pitahaya, perlite, tezontle, litter potting mix, rooting
Abstract:
At the Universidad Autónoma Metropolitana, plant tissue culture teaching requires species with a fast and effective response due to the limited courses duration and facilities. This situation is a challenge for us to find the suitable materials for our 11 week laboratory practices. We have developed a successful protocol for pitahaya micropropagation. In this paper, we report an experiment conducted to evaluate pitahaya shoots rooting and acclimatization ability in 3 agricultural substrata. There were no significant differences for both variables in perlite and tezontle treatments. Nevertheless rooting best results were obtained in tezontle (91.6%) while establishment were best in perlite (96.6%). Plant vigour were best in tezontle. The ability of this species for simultaneously be rooted and ex vitro adapted in four weeks allow us to offer a complete view of micropropagation and acclimatization process to our students.

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