ISHS Acta Horticulturae 748: II International Symposium on Acclimatization and Establishment of Micropropagated Plants
EX VITRO GROWTH OF TRANSFORMED PAPAYA PLANTS CONTAINING A BACTERIAL CITRATE SYNTHASE GENE |
| Authors: | F. Alatorre-Cobos, C. Talavera M., F. Espadas G., J.M. Santamarķa, A. O'Connor S. |
| Keywords: | acclimatization, gltA gene, citrate, phosphorus, photosynthesis |
| Abstract:
Several reports have shown that transgenic plants overproducing organic acids have a higher ability to uptake phosphorus than their non-transgenic controls. This is due to the fact that organic acids help dissolve phosphates from insoluble compounds in the rizhosphere. In the present study, papaya embryogenic calli transformed with a citrate synthase gene (gltA gene) from Pseudomonas aeruginosa were regenerated, micropropagated in vitro and acclimatized and established under greenhouse conditions. Plant growth was measured in two transformed lines and non-transformed plants. There were no differences in ex vitro establishment efficiency in both types of plants; however, 150 days after acclimatization, the plant growth in transformed plants was higher than in controls plants. This could be associated to an improvement in the acquisition of phosphorus and/or a higher photosynthetic rate found in transformed lines in early growth stages. | |
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