ISHS Acta Horticulturae 745: VI International Solanaceae Conference : Genomics Meets Biodiversity
CONSTRUCTION OF LIBRARIES OF 'MICRO-TOM' TOMATO MUTATIONS INDUCED BY HEAVY-ION BOMBARDMENT |
| Authors: | S. Imanishi, A. Noguchi, M. Nagata, T. Abe, I. Honda |
| Keywords: | fruit, surface color, carotenoid, flower, meristem |
| Abstract:
Fruit setting, development and ripening are complex, genetically programmed processes. Identifying the factors that control these processes is important for understanding the mechanisms of reproductive development. We induced mutations in the tomato cultivar ‘Micro-Tom’ by irradiation with accelerated heavy ions, recently established as an effective method for inducing mutations in plants, and constructed mutation libraries. Screening 2064 M1 plants, we found various mutations, including those affecting the sizes of plants, leaves, or fruit, and one conferring a broccoli-like inflorescence. To date, we have visually phenotyped 516 M2 families (five sibs per line) in the field, and found 60 plants differing from the wild type in one or more characteristics. The fruit of one was orange, and the beta-carotene content was increased at the expense of lycopene. These results suggest that such mutation libraries could be powerful tools to explain the reproductive development of plants. | |
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