ISHS Acta Horticulturae 744: I International Symposium on Human Health Effects of Fruits and Vegetables
EVALUATION OF FREE RADICAL SCAVENGING PROPERTIES OF GRAPE PHENOLIC EXTRACTS BY A FAST COLORIMETRIC METHOD |
| Authors: | S. Caillet, S. Salmieri, M. Lacroix |
| Keywords: | HPLC, diethyl-p-phenylenediamine |
| Abstract:
Three commercial extracts of grape phenols (seeds, skin, whole) were used in this study. Each extract was divided by HPLC into five fractions of different polarity, and their free radical scavenging activity was measured using the DPD (N,N-diethyl-p-phenylenediamine) colorimetric method. Total phenols content were determined using the Folin-Ciocalteu method to assess their contribution to the antiradical activity. The results showed that there was good correlation between total phenolic compounds contents and free radical scavenging activity of the different grape extracts. Moreover, the seed extract presented the greatest free radical scavenging properties (138 USP define USP/mg extract), whereas the whole grape extract had the lowest free radical scavenging capacity (80.5 USP/mg extract). However, the free radical scavenging properties reported on the basis of content of phenolic compounds in each extract showed that the free radical scavenging activity of seed and whole extracts were not significantly different (171 versus 162 USP/mg gallic acid equivalents phenol). In addition, free radical scavenging activity of fractions from seed and skin extracts decreased as their polarity decreased. | |
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