ISHS Acta Horticulturae 744: I International Symposium on Human Health Effects of Fruits and Vegetables
BREEDING THE AMERICAN CRANBERRY FOR HEALTH CONSTITUENTS: GENETIC SEPARATION OF GREEN TEA CATECHINS BY ELECTRODIALYSIS WITH ULTRAFILTRATION MEMBRANES |
| Authors: | D. Labbé, L. Bazinet, E. Vignal, P. Sistat |
| Keywords: | electromigration, ultrafiltration membrane, EGCG, catechins, separation, green tea |
| DOI: | 10.17660/ActaHortic.2007.744.41 |
| Abstract:
Numerous studies in the past years have highlighted the preventive and the curative potential action of green tea catechins on some diseases. Recently, a combination of electrodialysis (ED) with one ultrafiltration (UF) membrane stacked was demonstrated by Labbé et al. (2005) to separate green tea catechins, especially (-)-epigallocatechin gallate (EGCG) and (-)-epigallocatechin (EGC) (Fig. 1). In this work, a 50% decrease in EGCG and EGC in the green tea compartment was observed. The purpose of the present study was to demonstrate the effect of UF membrane pore sizes on the selective separation of the different green tea catechins and to quantify their migration into the adjacent KCl compartment. It appeared from this study that EGCG and EGC migrations of 50% in the green tea compartment was not confirmed in the KCl compartment. According to the pH evolution in the KCl compartment, EGCG and EGC migrations were stopped by the large acidification observed with the UF 500, 1000 and 5000 Da membranes that changed their -potential from negative to positive, and thus their electromigration potential. With the UF 10000 Da membrane, the high basification observed in the KCl compartment kept the electromigration potential of EGCG and EGC, but involves their degradation. | |
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